An experimental workflow for identifying RNA m6A alterations in cellular senescence by methylated RNA immunoprecipitation sequencing

N6-methyladenosine (m6A), the most prevalent mRNA modification in eukaryotic cells, is known to play regulatory roles a wide array of biological processes, including aging and cellular senescence. To investigate such roles, m6A can be identified across entire transcriptome by immunoprecipitation methylated RNA with an anti-m6A antibody, followed high-throughput sequencing (meRIP-seq or m6A-seq). Presented here protocol for employing meRIP-seq profile landscape senescent human cells. We described, detail, sample preparation, isolation, immunoprecipitation, library sequencing, bioinformatic analysis validation. also provided tips considerations optimization interpretation results. Our serves as methodological resource investigating transcriptomic alterations senescence well valuable paradigm validation genes interest.